Relationship between Gleason`s score and immunohistochemical staining intensity of tissue PSA in prostatic carcinoma

Abstract

Objective: To evaluate the usefulness of Immunohistochemical Staining of Tissue PSA in determining the severity of Prostatic Carcinoma. Material and Methods: An explanatory study carried out at the Department of Histopathology of a Tertiary Care Hospital Lahore, from January 1999 to July 2002. Sixty-two (62)- cases of prostatic adenocarcinoma were graded with Gleason`s score and stained with immunohistochemical stain for tissue PSA. Intensity of staining was correlated with Gleason`s score and relationship was evaluated by ANOVA and simple regression. Specificity & sensitivity of staining intensity was estimated for specific Gleason`s scores. Results: Age of the 62 cases ranged between 50 – 90 years and no significant difference was found between age groups regarding tumor cell differentiation. Staining intensity was found to be positively related to cellular differentiation (->2 51). `Relationship between staining intensity and Gleason`s score showed an inverse relationship with ANOVA / linear regression; F statistic of 209.72 (p.0001) and R 0.882. Conclusion: Our study confirmed the findings of other international studies exploring relationships between Gleason`s score and staining intensity of tissue PSA. Staining of tissue PSA with immunohistochemical stains is helpful in diagnosing the severity of the tumor grade as a complimentary tool along with Gleason`s scoring.